t75 tissue culture flasks with ventilation caps Search Results


95
Guangzhou JET Bio-Filtration t75 tissue culture flask
T75 Tissue Culture Flask, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Avantor t75 tissue culture flasks
T75 Tissue Culture Flasks, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences t-75 tissue culture flasks
T 75 Tissue Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
ATCC a549 t 75 cm2 flasks
A549 T 75 Cm2 Flasks, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Santa Cruz Biotechnology t75 flasks
T75 Flasks, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences noncoated corning t75 tissue culture flasks
Noncoated Corning T75 Tissue Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-One Inc t75 tissue culture flasks
T75 Tissue Culture Flasks, supplied by Bio-One Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc t75 tissue culture treated flask stemcell 200–0501
T75 Tissue Culture Treated Flask Stemcell 200–0501, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd tissue culture flasks
Tissue Culture Flasks, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson t75 flask
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Genesee Scientific flat bottom plate
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99
ATCC hcecs materials complete corneal epithelial media
Schematic depicting the protocol for <t>hCECs,</t> hCSSCs, and hiNSCs seeding onto silk scaffolds.
Hcecs Materials Complete Corneal Epithelial Media, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Schematic depicting the protocol for hCECs, hCSSCs, and hiNSCs seeding onto silk scaffolds.

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Schematic depicting the protocol for hCECs, hCSSCs, and hiNSCs seeding onto silk scaffolds.

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques:

Evaluating the effects of the microenvironment (elevated glucose or dynamic perfusion) and different chemical stimulants (capsaicin and sodium lauryl sulfate) on cell viability and gene and protein expression using the corneal tissue model. ( Abbreviations : Interleukin-1 beta (IL-1β), Substance P (SP), Calcitonin gene-related peptide (CGRP); Gene names : CRCP -accessory protein for CGRP, SCN- sodium channel, KERA - keratocan (cornea-specific proteoglycan), LUM - lumican (proteoglycan abundant in the stroma)).

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Evaluating the effects of the microenvironment (elevated glucose or dynamic perfusion) and different chemical stimulants (capsaicin and sodium lauryl sulfate) on cell viability and gene and protein expression using the corneal tissue model. ( Abbreviations : Interleukin-1 beta (IL-1β), Substance P (SP), Calcitonin gene-related peptide (CGRP); Gene names : CRCP -accessory protein for CGRP, SCN- sodium channel, KERA - keratocan (cornea-specific proteoglycan), LUM - lumican (proteoglycan abundant in the stroma)).

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques: Expressing, Concentration Assay, Permeability

Common secreted proteins related to nerve activation or tissue inflammation present in conditioned media isolated from corneal constructs. The relative concentration ranges of each protein detected in conditioned media in response to various chemical stimuli have been previously reported by our group ( Deardorff et al., 2018 ; Siran et al., 2018 ). ( Abbreviations : Substance P (SP), Calcitonin gene-related peptide (CGRP), Interleukin-1 beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), Matrix metalloproteinase-9 (MMP-9)).

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Common secreted proteins related to nerve activation or tissue inflammation present in conditioned media isolated from corneal constructs. The relative concentration ranges of each protein detected in conditioned media in response to various chemical stimuli have been previously reported by our group ( Deardorff et al., 2018 ; Siran et al., 2018 ). ( Abbreviations : Substance P (SP), Calcitonin gene-related peptide (CGRP), Interleukin-1 beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), Matrix metalloproteinase-9 (MMP-9)).

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques: Activation Assay, Isolation, Construct, Concentration Assay

Phenotypic markers of each cell type present in the corneal construct. ( Abbreviations : Transient receptor potential cation channel subfamily V member 1 (TRPV1), Transient receptor potential cation channel subfamily A member 1 (TRPA1), Transient receptor potential cation channel subfamily M member 8 (TRPM8)).

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Phenotypic markers of each cell type present in the corneal construct. ( Abbreviations : Transient receptor potential cation channel subfamily V member 1 (TRPV1), Transient receptor potential cation channel subfamily A member 1 (TRPA1), Transient receptor potential cation channel subfamily M member 8 (TRPM8)).

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques: Construct, Marker

Troubleshooting guide for construct assembly and characterization.

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Troubleshooting guide for construct assembly and characterization.

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques: Construct, Cell Culture, Microscopy, Incubation, Sterility, Isolation, Lysis, Homogenization, Binding Assay

Relative timeframe for completion of each step in the assembly, stimulation, and characterization of the corneal tissue model. The amount of time may vary significantly depending on the user and the total number of constructs generated. These timeframes are based on 12 constructs per experiment.

Journal: Current protocols in toxicology

Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide

doi: 10.1002/cptx.84

Figure Lengend Snippet: Relative timeframe for completion of each step in the assembly, stimulation, and characterization of the corneal tissue model. The amount of time may vary significantly depending on the user and the total number of constructs generated. These timeframes are based on 12 constructs per experiment.

Article Snippet: SUBCULTURING OF hCECs Materials Complete corneal epithelial media ( Refer to Reagents and Solutions Section ) Pipette T75 flask Primary human corneal epithelial cells (hCECs, ATCC, cat. no. ATCC® PCS-700-010TM) FNC Coating Mix® (Athena Environmental Sciences, cat. no. 0407) (Optional) 500 mL cell culture flask Seed and maintain hCECs 1.

Techniques: Construct, Generated, Incubation, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay, Imaging, Lysis, Protein Concentration, BIA-KA, Western Blot