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Guangzhou JET Bio-Filtration
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Avantor
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Corning Life Sciences
t-75 tissue culture flasks T 75 Tissue Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/t75+tissue+culture+flasks+with+ventilation+caps/96+well+plates/10__1042_slash_bj3170371-12-33-37 Average 90 stars, based on 1 article reviews
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ATCC
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Santa Cruz Biotechnology
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Corning Life Sciences
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Bio-One Inc
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STEMCELL Technologies Inc
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Valiant Co Ltd
tissue culture flasks Tissue Culture Flasks, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/t75+tissue+culture+flasks+with+ventilation+caps/Cell+Culture+Flasks/pmc03136433-400-20-44 Average 93 stars, based on 1 article reviews
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Becton Dickinson
t75 flask T75 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/t75+tissue+culture+flasks+with+ventilation+caps/24+well+plates/pmc04399413-46-11-13 Average 90 stars, based on 1 article reviews
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Genesee Scientific
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ATCC
hcecs materials complete corneal epithelial media ![]() Hcecs Materials Complete Corneal Epithelial Media, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/t75+tissue+culture+flasks+with+ventilation+caps/Primary+Corneal+Epithelial+Cells%3B+Normal%2C+Human/pmc06754119-1503-2-25 Average 99 stars, based on 1 article reviews
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Image Search Results
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Schematic depicting the protocol for hCECs, hCSSCs, and hiNSCs seeding onto silk scaffolds.
Article Snippet: SUBCULTURING OF
Techniques:
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Evaluating the effects of the microenvironment (elevated glucose or dynamic perfusion) and different chemical stimulants (capsaicin and sodium lauryl sulfate) on cell viability and gene and protein expression using the corneal tissue model. ( Abbreviations : Interleukin-1 beta (IL-1β), Substance P (SP), Calcitonin gene-related peptide (CGRP); Gene names : CRCP -accessory protein for CGRP, SCN- sodium channel, KERA - keratocan (cornea-specific proteoglycan), LUM - lumican (proteoglycan abundant in the stroma)).
Article Snippet: SUBCULTURING OF
Techniques: Expressing, Concentration Assay, Permeability
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Common secreted proteins related to nerve activation or tissue inflammation present in conditioned media isolated from corneal constructs. The relative concentration ranges of each protein detected in conditioned media in response to various chemical stimuli have been previously reported by our group ( Deardorff et al., 2018 ; Siran et al., 2018 ). ( Abbreviations : Substance P (SP), Calcitonin gene-related peptide (CGRP), Interleukin-1 beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), Matrix metalloproteinase-9 (MMP-9)).
Article Snippet: SUBCULTURING OF
Techniques: Activation Assay, Isolation, Construct, Concentration Assay
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Phenotypic markers of each cell type present in the corneal construct. ( Abbreviations : Transient receptor potential cation channel subfamily V member 1 (TRPV1), Transient receptor potential cation channel subfamily A member 1 (TRPA1), Transient receptor potential cation channel subfamily M member 8 (TRPM8)).
Article Snippet: SUBCULTURING OF
Techniques: Construct, Marker
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Troubleshooting guide for construct assembly and characterization.
Article Snippet: SUBCULTURING OF
Techniques: Construct, Cell Culture, Microscopy, Incubation, Sterility, Isolation, Lysis, Homogenization, Binding Assay
Journal: Current protocols in toxicology
Article Title: Assembly and Application of a 3D Human Corneal Tissue Model: A Step-by-Step Guide
doi: 10.1002/cptx.84
Figure Lengend Snippet: Relative timeframe for completion of each step in the assembly, stimulation, and characterization of the corneal tissue model. The amount of time may vary significantly depending on the user and the total number of constructs generated. These timeframes are based on 12 constructs per experiment.
Article Snippet: SUBCULTURING OF
Techniques: Construct, Generated, Incubation, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay, Imaging, Lysis, Protein Concentration, BIA-KA, Western Blot